The phases that can be distributed are limited to the solid phase and the liquid phase

According to the molecular size and shape, it is divided into methods such as differential centrifugation, ultrafiltration, molecular sieve, and dialysis; according to solubility, it is divided into methods such as salting out, solvent extraction, partition chromatography, countercurrent distribution and crystallization; according to the difference in charge, it is divided into electrophoresis, electric Dialysis, isoelectric point deposition, ion exchange chromatography and adsorption chromatography, etc. 

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At the same time, attention should be paid to avoid the pollution of heavy metal ions, cell enzymes and other toxic substances in the system. Therefore, it is difficult to have a uniform standard method that can be used for any protein.u3000u3000 

The entire preparation process can generally be divided into 5 stages: selection and pretreatment of data, breaking of cells (sometimes separation of organelles), extraction, purification (including salting out, organic solvent deposition, organic solvent extraction, adsorption, chromatography, ultracentrifugation) And crystallization, etc. The preparation method can be classified according to the primary factors such as molecular size, shape, charged properties and solubility. For example, the proteolytic enzyme activity of the crude

 venom of agkistrodon is very high, which needs attention in the separation and purification. In addition, the polymer protein has a certain three-dimensional conformation, which is

 appropriately unstable, and is extremely easy to be denatured and allosteric as described above, which limits the methods of separation and purification. the above The five stages do not require that every plan be fully equipped, nor is each stage completely separated. Because the protein caot be dissolved or evaporated, the phases that can be distributed are limited to the solid phase and the liquid phase, and the two phases replace each other for separate purification